About DDNS
We have developed laboratory protocols for the direct detection and nanopore sequencing (DDNS) of poliovirus directly from stool and environmental samples in response to the need for faster diagnostics and improved biosafety compared with cell culture. DDNS has been implemented in four WHO regions, allowing faster polio outbreak detection and in-house generation of poliovirus sequence data. DDNS is currently under review by the WHO for routine implementation as an accepted Global Polio Laboratory Network (GPLN) method for stool sample testing.
We have also adapted the DDNS protocols for sequencing of cell culture isolates in laboratories that previously did not have this capacity. This has helped support the expansion of sequencing capacity across the GPLN. We continue to provide technical support for DDNS to the GPLN and strive for continual innovation and improvement of polio diagnostic and surveillance tools.
Learn more
All the protocols, quality control steps and bioinformatic resources are available from protocols.io, just click the link below.